The 3D structure determination of proteins using X-ray Diffraction is one of the core services of our Infrastructure. Knowledge of the 3D structure of macromolecular targets of pharmaceutical and biotechnological interest and their complexes with small molecules or other proteins may reveal the structure-function relationships that dictate their mechanism of action. Proteins participate in a plethora of metabolic pathways and controlling their interactions with their counterparts or bioactive compounds may allow the development of therapeutic interventions with significant socioeconomic impact.
Potential users: Scientists working in academic/research organizations, health sciences, hospitals, industries (pharmaceutical, food, agricultural, biofuel, paper, chemical) and SMEs.
Visit the provider's website: http://inspired.aua.gr/instruct/
Server consists of 24 units of 8-core Intel Xeon 2.8GHz workstations with 32GB internal memory each.
The D8 Venture system includes:
IμS microfocus X-ray source, Cu radiation, air cooled
Four-circle goniometer, computer-controlled, crystal-detector distance = 35-240mm, sphere of confusion of less than 7 micrometer
Detector PHOTON 100, CMOS technology, air-cooled, 100 x 100mm active area, 240e/Cu sensitivity, shutterless data collection
800 Series Cryostream cooler, Oxford Cryosystems, AD51 Dry Air Unit, Integrated Autofill system, 60 lt and 150 lt dewar vessels.
APEX3 and PROTEUM software
Professor Elias Eliopoulos is the Director of the Genetics Laboratory at the Biotechnology Department of the Agricultural University of Athens (A.U.A.) and leads the bioinformatics and structural biology group. He is professor of Biochemistry, a biophysicist and crystallographer by training with considerable experience in biomolecular structure analysis, epitope mapping of protein receptors, genetic polymorphisms and variation, cloning, with experimental and computational methods, protein structure prediction, ligand and drug design, protein design, in silico antibody design and biosoftware development. The group has experience and international reputation on computational protein folding, ab initio and homology modeling of proteins derived from edge gene research and membrane protein modeling. The group is also involved in many ligand computational design projects for pharmaceutical, medicinal and agricultural applications.
Server consists of 24 units of 8-core Intel Xeon 2.8GHz workstations with 32GB internal memory each.
The D8 Venture system includes:
IμS microfocus X-ray source, Cu radiation, air cooled
Four-circle goniometer, computer-controlled, crystal-detector distance = 35-240mm, sphere of confusion of less than 7 micrometer
Detector PHOTON 100, CMOS technology, air-cooled, 100 x 100mm active area, 240e/Cu sensitivity, shutterless data collection
800 Series Cryostream cooler, Oxford Cryosystems, AD51 Dry Air Unit, Integrated Autofill system, 60 lt and 150 lt dewar vessels.
APEX3 and PROTEUM software