Hellenic Pasteur Institute  |   UV-Vis spectroscopy
 
DESCRIPTION

UV-Vis spectroscopic measurements consist one of the most widely used methods for protein quantification. This is due to the fact that the tryptophan and tyrosine (and to a lesser extent the phenylalanine) residues of proteins strongly absorb at 280 nm. Hence, the corresponding absorbance is used for the calculation of protein concentration, based on specific quantification formulas. If the protein sample does not have tryptophan or tyrosine, the concentration can still be easily measured by the absorption at 205 nm in which the peptide bonds are analysed directly (Scopes method). Conformational studies can also be performed under special conditions.

Hellenic Pasteur Institute
LOCATION:   Athens, Greece | https://www.pasteur.gr/en/
RELEVANT STAFF:   Stathis Kotsakis , Vivi Miriagou
SERVICE DETAILS:  

A double beam UV-Vis spectrophotometer (HITACHI U-2001) is available in the HPI’s Bacteriology laboratory measuring absorbance at the range 190 – 1100 nm with temperatures being controlled trough a water circulation apparatus. The device can be used for a variety of applications such as protein and nucleic acid quantification, wave length scans, and time scans permitting the real time monitoring of enzymatic reactions for steady state kinetic analyses and inhibition studies. The instrument is PC controlled and the relevant analyses can be performed with the UV Visions software (Hitachi).

RELEVANT EQUIPMENT:  
EQUIPMENT UNIT:  
U-2001 by Hitachi
TYPE:   Spectrophotometer
ADDITIONAL INFO:  
 
LOCATION:   Building 8 (3rd floor) HPI
DESCRIPTION:  
FUNDER:  
 
 
 
RELEVANT EXPERTISE: