Sample preparation  |   Purification chromatographic system
 
DESCRIPTION
The description of this service is not available.
NATIONAL HELLENIC RESEARCH FOUNDATION (N.H.R.F.)
LOCATION:   Athens, Greece | http://www.eie.gr/index-en.html
RELEVANT STAFF:   Spyros Zographos , Evangelia Chrysina
SERVICE DETAILS:  

 

RELEVANT EQUIPMENT:  
EQUIPMENT UNIT:  
ÄKTApurifier UPC 10 by GE Healthcare
TYPE:   Fast Liquid Protein Chromatography apparatus
ADDITIONAL INFO:  
 
LOCATION:   Institute of Chemical Biology- Laboratory of Structural Biology / Instruct-EL hub
DESCRIPTION:   Fast Liquid Chromatography apparatus for protein purification
FUNDER:   OPERATIONAL PROGRAMME "COMPETITIVENESS", Measure 4.5., Action 4.5.1.: INFRASTRUCTURE TO SUPPORT THE LONG TERM VIABILITY OF RESEARCH ACTIVITIES, TECHNOLOGICAL PROMOTION AND DISSEMINATION. Project title: DESIGN AND SYNTHESIS OF BIOACTIVE COMPOUNDS; FP7-REGPOT-2009-1, No245866, "ARCADE"
 
 
RELEVANT EXPERTISE:  
NATIONAL CENTRE FOR SCIENTIFIC RESEARCH "DEMOKRITOS", NCSR-DEMOKRITOS
LOCATION:   Athens | http://www.demokritos.gr/
RELEVANT STAFF:   Angeliki Chroni
SERVICE DETAILS:   Not available.
RELEVANT EQUIPMENT:  
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Purifier by Acta
TYPE:   FPLC
ADDITIONAL INFO:  
 
LOCATION:   NCSRD, Building 20
DESCRIPTION:  
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RELEVANT EXPERTISE:  
HELLENIC PASTEUR INSTITUTE
LOCATION:   Athens, Greece | https://www.pasteur.gr/en/
SERVICE DETAILS:  

At the Hellenic Pasteur Institute the procedure of chromatographic analysis by use of Fast Purification Liquid Chromatographic (FPLC) systems has been implemented. A wide range of resins can be used, including affinity, size-exclusion and ion exchange chromatography. For further analysis of the isolated macromolecules, protein electrophoretic systems are also available.

RELEVANT EQUIPMENT:  
EQUIPMENT UNIT:  
AKTAPurifier UPC10 by Amersham-Pharmacia
TYPE:   Fast protein liquid chromatography system
ADDITIONAL INFO:  
 
LOCATION:   Building 6 (groundfloor) HPI
DESCRIPTION:   Chromatography system for protein purification
FUNDER:   ΕΣΠΑ-ΕΚΒΑΝ/Inspired
 
 
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Various chromatographic cartridges by
TYPE:   Chromatographic cartridges
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LOCATION:  
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S-30 Elmasonic by Elma
TYPE:   Ultrasonic cleaning unit
ADDITIONAL INFO:  
 
LOCATION:   Building 6 (groundfloor) HPI
DESCRIPTION:  
FUNDER:   HPI
 
 
EQUIPMENT UNIT:  
AKTAPurifier 10 by Amersham-Pharmacia
TYPE:   Fast protein liquid chromatography system
ADDITIONAL INFO:  
 
LOCATION:   Building 6 (groundfloor) HPI
DESCRIPTION:   Chromatography system for Protein purification
FUNDER:   HPI/Inspired
 
 
RELEVANT EXPERTISE:  
NATIONAL TECHNICAL UNIVERSITY OF ATHENS
LOCATION:   Athens | https://www.ntua.gr/en/
SERVICE DETAILS:  

NTUA is  a fully equipped lab in terms of protein isolation and purification. The relative equipment includes a customizable modular purification system (pump, mixer, UV detector and fraction collector), which can be adapted to the desired application. The lab also provides a wide array of diffrent separation resins, including ion exchange chromatography, hydrophobic interactions chromatography, gel filtration chromatography, and affinity chromatography. 

Protein electrophoresis systems (both SDS-PAGE electrophoresis and IEF), together with ultrafiltration devices, are also available. Overall, the NTUA lab can fully support users aiming to isolate target proteins from microbial culture supernatants, either from native or heterologous microbial hosts, and provide the suitable equipment and expertise to assess the purity of the resulting protein samples, and their quality for subsequent structural studies.  

RELEVANT EQUIPMENT:  
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Fraction collector SF-2120 by Advantec
TYPE:   Fraction collector
ADDITIONAL INFO:  
 
LOCATION:   NTUA
DESCRIPTION:  

Fraction collector for protein purification. The ASVANTEC Super Fraction Collector has the ability to collect microliter to liter size fraction directly into a variety of containers. The standard test tube rack,which doubles as an ice bath,can collect up to 120 fractions in test tubes. The SF-2120 has a height adjustment system that allows you to use test tubes or bottles up to 180 mm in height. The self adjusting tapered test tube rack can accommodate test tubes from 12 mm to 18 mm in diameter. Bottles can be organized in the test tube tray and large volumes can be collected using the "Bottle Mode"


FUNDER:  
 
 
EQUIPMENT UNIT:  
Mini-PROTEAN 3 cell by Biorad
TYPE:   Protein electrophoresis system
ADDITIONAL INFO:  
 
LOCATION:   NTUA
DESCRIPTION:  

Mini-PROTEAN 3 Electrophoresis System, 10 well, 0.75 and 1.50 thickness, complete system includes combs, glass plates, casting stand, casting frames, and Electrophoresis Module


FUNDER:  
 
 
EQUIPMENT UNIT:  
Amicon 8000 by Merck
TYPE:   Ultrafiltration system - 400ml
ADDITIONAL INFO:  
 
LOCATION:   NTUA
DESCRIPTION:  

Amicon® Stirred Cells

Pressure-based sample concentration

Amicon® stirred cells are ideal for finely separating macrosolutes (DNA, RNA, protein, etc.) in large-volume (400 mL) samples. They are also reliable tools for membrane analysis, because they allow users to insert membranes of choice. Stirred cells concentrate solutions with up to 10% initial macrosolute concentration with high flow rates and high recovery. They can be also used for salt removal followed by concentration in the same unit, and complete product recoveries can generally be achieved.

Gentle magnetic stirring minimizes concentration polarization and shear denaturation. All stirred cells can be autoclaved. 


FUNDER:  
 
 
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Econo Gradient pump and mixer by BioRad
TYPE:   Protein purification system
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LOCATION:  
DESCRIPTION:  

Peristaltic pump and mixer for up to two solvents gradient. Manual and programmed operation.


FUNDER:  
 
 
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EM-1 Econo UV Monitor by Biorad
TYPE:   UV detector
ADDITIONAL INFO:  
 
LOCATION:   NTUA
DESCRIPTION:  

The Model EM-1 Econo UV monitor is a single-wavelength detector for flowthrough monitoring of effluents from chromatographic columns, centrifugation gradients, and other devices. The monitor consists of a control unit and an optics module that includes both 254 and 280 nm filters and a 2 mm pathlength flow cell. Connection is via Luer-Lok fittings for quick, easy changes. Key features include:

Portable optics module with detection close to the column outlet to maximize resolution

Self-contained filters

Autozeroing function

Coldroom compatibility

LED display of absorbance


FUNDER:   INSPIRED (MIS 5002550)
 
 
RELEVANT EXPERTISE:  
AGRICULTURAL UNIVERSITY OF ATHENS
LOCATION:   Athens | https://www2.aua.gr/en
RELEVANT STAFF:   Elias Eliopoulos
SERVICE DETAILS:  

Visit the provider's website: http://inspired.aua.gr/instruct/

RELEVANT EQUIPMENT:  
EQUIPMENT UNIT:  
ÄKTA™ start by GE Healthcare Life Sciences
TYPE:   Fast Protein Liquid Chromatography apparatus
ADDITIONAL INFO:  
 
LOCATION:   75 Iera Odos, Athens 118 55, Greece, Agricultural University of Athens, Hassiotes Build., 1st Floor, Biotechnology Dept., Genetics Lab
DESCRIPTION:  

Chromatography system for Protein purification


FUNDER:   INSPIRED-AUA GSRT
 
 
RELEVANT EXPERTISE:  
UNIVERSITY OF IOANNINA
LOCATION:   Ioannina, Greece | https://www.uoi.gr/en/
RELEVANT STAFF:   Anastasia Politou
SERVICE DETAILS:   Not available.
RELEVANT EQUIPMENT:  
EQUIPMENT UNIT:  
FPLC system ActaPurifier by GE Life Sciences
TYPE:   Fast Liquid Protein Chromatography
ADDITIONAL INFO:  
 
LOCATION:   Laboratory of Biological Chemistry, Faculty of Medicine, University of Ioannina, Ioannina, Greece
DESCRIPTION:   FPLC system equipped with several columns and resins.
FUNDER:  
 
 
RELEVANT EXPERTISE:  
UNIVERSITY OF CRETE
LOCATION:   Heraklion, Crete | http://en.uoc.gr/
RELEVANT STAFF:   Kathrin Kefala
SERVICE DETAILS:  

Akta purifier from GE Healthcare is used for the protein purification with simple column selection. Its features are:

Automatic titration of buffers across a broad pH range

Quick and easy transfer of methods to production-scale

UNICORN method wizard High performance purification and characterization

State-of-the-art computer-controlled pumps, UV/conductance monitors, and fraction collector

UV-Vis detection of up to three wavelengths simultaneous

RELEVANT EQUIPMENT:  
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Chromatography system by GE Healthcare-Sweden
TYPE:  
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