At the Hellenic Pasteur Institute the procedure of chromatographic analysis by use of Fast Purification Liquid Chromatographic (FPLC) systems has been implemented. A wide range of resins can be used, including affinity, size-exclusion and ion exchange chromatography. For further analysis of the isolated macromolecules, protein electrophoretic systems are also available.
NTUA is a fully equipped lab in terms of protein isolation and purification. The relative equipment includes a customizable modular purification system (pump, mixer, UV detector and fraction collector), which can be adapted to the desired application. The lab also provides a wide array of diffrent separation resins, including ion exchange chromatography, hydrophobic interactions chromatography, gel filtration chromatography, and affinity chromatography.
Protein electrophoresis systems (both SDS-PAGE electrophoresis and IEF), together with ultrafiltration devices, are also available. Overall, the NTUA lab can fully support users aiming to isolate target proteins from microbial culture supernatants, either from native or heterologous microbial hosts, and provide the suitable equipment and expertise to assess the purity of the resulting protein samples, and their quality for subsequent structural studies.
Fraction collector for protein purification. The ASVANTEC Super Fraction Collector has the ability to collect microliter to liter size fraction directly into a variety of containers. The standard test tube rack,which doubles as an ice bath,can collect up to 120 fractions in test tubes. The SF-2120 has a height adjustment system that allows you to use test tubes or bottles up to 180 mm in height. The self adjusting tapered test tube rack can accommodate test tubes from 12 mm to 18 mm in diameter. Bottles can be organized in the test tube tray and large volumes can be collected using the "Bottle Mode"
Mini-PROTEAN 3 Electrophoresis System, 10 well, 0.75 and 1.50 thickness, complete system includes combs, glass plates, casting stand, casting frames, and Electrophoresis Module
Amicon® Stirred Cells
Pressure-based sample concentration
Amicon® stirred cells are ideal for finely separating macrosolutes (DNA, RNA, protein, etc.) in large-volume (400 mL) samples. They are also reliable tools for membrane analysis, because they allow users to insert membranes of choice. Stirred cells concentrate solutions with up to 10% initial macrosolute concentration with high flow rates and high recovery. They can be also used for salt removal followed by concentration in the same unit, and complete product recoveries can generally be achieved.
Gentle magnetic stirring minimizes concentration polarization and shear denaturation. All stirred cells can be autoclaved.
Peristaltic pump and mixer for up to two solvents gradient. Manual and programmed operation.
The Model EM-1 Econo UV monitor is a single-wavelength detector for flowthrough monitoring of effluents from chromatographic columns, centrifugation gradients, and other devices. The monitor consists of a control unit and an optics module that includes both 254 and 280 nm filters and a 2 mm pathlength flow cell. Connection is via Luer-Lok fittings for quick, easy changes. Key features include:
Portable optics module with detection close to the column outlet to maximize resolution
LED display of absorbance
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Chromatography system for Protein purification
Akta purifier from GE Healthcare is used for the protein purification with simple column selection. Its features are:
Automatic titration of buffers across a broad pH range
Quick and easy transfer of methods to production-scale
UNICORN method wizard High performance purification and characterization
State-of-the-art computer-controlled pumps, UV/conductance monitors, and fraction collector
UV-Vis detection of up to three wavelengths simultaneous