High-yield expression system based on transfected mammalian cells for the production of recombinant proteins with complex post-translational modifications. Apart from the available adherent cells, our Institute has the expertise in using the highly sophisticated system of mammalian Expi293F cells which ensures rapid and high-yield protein production from high density suspension culture. The culture's supernatant is filtrated and then concentrated/dialysed by use of tangential flow filtration systems. The subsequent isolation of the recombinant proteins is achieved through chromatographic analysis.
Some des
BIOBASE Biochemistry incubator, BIOBASE Biological Safety Cabinet Class II A2, Euromex Inverted microscope (OX.2053-PLP, euromex)
Senior Research Scientist, Department of Molecular Biology and Genetics, Democritus University of Thrace.
Education
BSc, Department of Biology, University of Patras, Greece (1992)
PhD, Department of Chemistry, University of Patras, Greece (1999)
Work experience
Post-doctoral fellow, Laboratory of Protein Chemistry, Department of Pathology & Lab. Med., University of Pennsylvania, Philadelphia, Pennsylvania (1999-2000)
Post-doctoral research scientist, Department of Medicine, Division of Hematology and Oncology, State University of New York at Stony Brook, New York (2000-2003)
Post-doctoral research associate, Laboratory of Neurobiology of Degenerative Diseases, Foundation of Biomedical Research of the Academy of Athens, Greece (2004-2008)
Teaching of Biochemistry (assistant professor level), Department of Bioinformatics, University of Central Greece, Lamia, Greece (2009)
Senior research scientist, CiBit Facility, Molecular Interactions Unit, Department of Molecular Biology and Genetics, Democritus University of Thrace (December 2010- 2012).
Post-doctoral research scientist, Department of Medicine, Division of cardiology, Democritus University of Thrace (2012-2020).
Experience related to current position.
As responsible for the molecular interaction unit starting on 2010, I gained expertise on SPR technology running several projects on ProteOn XPR36 biosensor (BIORAD), most of them published in peer reviewed journals. Following end of funding for this position on 2012, I continued running projects on the biosensor in collaboration with internal researchers from University of Thrace as well as other Greek research centers until 2016, as the most expertised user.
Dr. Katerina R. Katsani is Assistant Professor at the Dept of Molecular Biology & Genetics in the Democritus University of Thrace. Dr. Katsani holds a Chemistry degree and a Μaster degree from the Dept of Chemistry of the Aristroteleion University in Thessaloniki. She did her PhD in London (CRUK, former ICRF) and Leiden University Medical Center in the Netherlands from where she obtained her PhD in 2002. During her PhD, she studied chromatin regulators in Drosophila. She then moved to Institut Curie in Paris as a Human Frontiers (HFSPO) post doctoral fellow where she worked on live imaging of nuclear pore complexes. Her main research interests focus on the characterization of the Drosophila nuclear pore complexes, and the investigation of the role of nuclear pore proteins in cell physiology using imaging and proteomic approaches.
BIOBASE Biochemistry incubator, BIOBASE Biological Safety Cabinet Class II A2, Euromex Inverted microscope (OX.2053-PLP, euromex)