The expression system of Leishmania tarentolae is an easy to use expression system that does not require any special conditions. The user is able to cultivate cells in biosafety level 2 conditions, at 25o-26o C in either static or stirring incubator, following given instructions.
At the Hellenic Pasteur Institute, we are able to provide plasmids for the expression of proteins in Leishmania tarentolae cells, as well as guidance for the correct cloning of genes of interest into the appropriate plasmid, for the precise transfection and characterization of Leishmania tarentolae strains and for the cell culture protocols. The service is limited only to providing experimental protocols and advices for the development of the L. tarentolae strain expressing the protein of interest.
We have eight years of experience in using the Leishmania tarentolae parasite for expressing heterologous as well as homologous recombinant proteins and developing transgenic strains. Latest publication: https://doi.org/10.1016/j.bbamem.2019.07.001