Biophysical characterization  |   Crystallization
 
DESCRIPTION
The description of this service is not available.
NATIONAL HELLENIC RESEARCH FOUNDATION (N.H.R.F.)
LOCATION:   Athens, Greece | http://www.eie.gr/index-en.html
RELEVANT STAFF:   Spyros Zographos , Evangelia Chrysina
SERVICE DETAILS:  

 

RELEVANT EQUIPMENT:  
EQUIPMENT UNIT:  
Leica ΜΖ16 by Leica Microsystems
TYPE:   Stereoscope
ADDITIONAL INFO:  
 
LOCATION:   Institute of Chemical Biology- X-ray Protein Crystallography facility "Nikos G. Oikonomakos" / Instruct-EL hub
DESCRIPTION:   Stereoscope equipped with a digital camera and polarized light (160x zoom)
FUNDER:   OPERATIONAL PROGRAMME "COMPETITIVENESS", Measure 4.5., Action 4.5.1.: INFRASTRUCTURE TO SUPPORT THE LONG TERM VIABILITY OF RESEARCH ACTIVITIES, TECHNOLOGICAL PROMOTION AND DISSEMINATION.
 
 
EQUIPMENT UNIT:  
OryxNano, Automatic Protein Crystallization Systems by Douglas Instruments
TYPE:   Protein Crystallisation Robot
ADDITIONAL INFO:  
 
LOCATION:   Institute of Chemical Biology- X-ray Protein Crystallography facility "Nikos G. Oikonomakos" / Instruct-EL hub
DESCRIPTION:   High-throughput macromolecular crystallization robot (3rd generation). (Installed in a controlled-temperature crystallization room, equipped with incubators in temperatures 10, 14, 16 and 20 °C)
FUNDER:   OPERATIONAL PROGRAMME "COMPETITIVENESS", Measure 4.5., Action 4.5.1.: INFRASTRUCTURE TO SUPPORT THE LONG TERM VIABILITY OF RESEARCH ACTIVITIES, TECHNOLOGICAL PROMOTION AND DISSEMINATION.
 
 
EQUIPMENT UNIT:  
Crystal Hotel by FORMULATRIX
TYPE:   Crystal Hotel
ADDITIONAL INFO:  
 
LOCATION:   Institute of Chemical Biology, NHRF
DESCRIPTION:  

Automated system for the detection, imaging and storage of protein crystal images grown under controlled temperature, ROCK IMAGER® 


FUNDER:   INSPIRED- MIS:5002550, EPANEK 2014-2020
 
 
RELEVANT EXPERTISE:  
UNIVERSITY OF PATRAS
LOCATION:   Patras, Greece | http://www.upatras.gr/en
RELEVANT STAFF:   Georgios Spyroulias
SERVICE DETAILS:   Not available.
RELEVANT EQUIPMENT:  
EQUIPMENT UNIT:  
Oryx Nano by Douglas Instruments
TYPE:   Crystallization Robot
ADDITIONAL INFO:  
 
LOCATION:   School of Pharmacy
DESCRIPTION:  
FUNDER:   EU
 
 
RELEVANT EXPERTISE:  
NATIONAL CENTRE FOR SCIENTIFIC RESEARCH "DEMOKRITOS", NCSR-DEMOKRITOS
LOCATION:   Athens | http://www.demokritos.gr/
RELEVANT STAFF:   Georgios Nounesis
SERVICE DETAILS:   Not available.
RELEVANT EQUIPMENT:  
EQUIPMENT UNIT:  
by
TYPE:  
ADDITIONAL INFO:  
 
LOCATION:   "Demokritos", Building 20
DESCRIPTION:  
FUNDER:   Inspired
 
 
RELEVANT EXPERTISE:  
HELLENIC PASTEUR INSTITUTE
LOCATION:   Athens, Greece | https://www.pasteur.gr/en/
SERVICE DETAILS:  

The facility has a variety of commercial crystallisation screening kits and incubators at 10 °C (Raypa FKS 1800) and 18 °C in a dedicated, temperature controlled room. Crystallization plates are set with an Oryx4 crystallization robot which provides the opportunity of screening a great range of crystallization conditions saving time and sample quantity (https://www.douglas.co.uk/oryx4.htm). Crystallisation conditions can be screened in 96-well plates (SWISSCI preferably, sitting drops only) in hundreds of nonoliter volumes. Screening for sitting drop experiments is carried out using plates that are prefilled with reservoir solutions by the users (reservoirs can be filled manually with a 8-channel pipette). Crystallisation trials are monitored with a Leica 12.5M stereoscope.

RELEVANT EQUIPMENT:  
EQUIPMENT UNIT:  
Oryx4 by Douglas Instruments
TYPE:   Protein crystallization robot
ADDITIONAL INFO:  
 
LOCATION:   Building 6 (2nd floor) HPI
DESCRIPTION:   Protein Crystallization Robot for sitting drop and microbatch screening with (simple) optimization.
FUNDER:   HPI
 
 
EQUIPMENT UNIT:  
Crystallization incubator by Raypa
TYPE:   Crystallization incubator
ADDITIONAL INFO:  
 
LOCATION:   Building 6 (2nd floor) HPI
DESCRIPTION:  
FUNDER:   HPI
 
 
RELEVANT EXPERTISE:  
AGRICULTURAL UNIVERSITY OF ATHENS
LOCATION:   Athens | https://www2.aua.gr/en
RELEVANT STAFF:   Elias Eliopoulos , Kostas Bethanis
SERVICE DETAILS:  

The facility has a variety of commercial crystallisation screening kits and incubators at 4 °C (Fiocchetti Medica 300) and 18 °C (FOC225i, VELP Scientifica). Crystallisation conditions can be screened either in 96-well plates or in 24-well plates in milliliter droplets. Crystallisation trials are monitored, visualized over time with Euromex Z-range by Euromex and Zeiss™ Stemi 2000-C & SV6 Stereo Microscopes and crystal images of high quality can be obtained with digital Canon camera.

Visit the provider's website: http://inspired.aua.gr/instruct/

RELEVANT EQUIPMENT:  
EQUIPMENT UNIT:  
Medica 300 by Fiocchetti
TYPE:   Refrigerated Incubator
ADDITIONAL INFO:  
 
LOCATION:   75 Iera Odos, Athens 118 55, Greece, Agricultural University of Athens, Hassiotes Build., 1st Floor, Biotechnology Dept., Physics Lab
DESCRIPTION:  
FUNDER:   Physics Lab, AUA
 
 
EQUIPMENT UNIT:  
Euromex Z-range by Euromex
TYPE:   Stereo microscope equipped with digital camera
ADDITIONAL INFO:  
 
LOCATION:   75 Iera Odos, Athens 118 55, Greece, Agricultural University of Athens, Hassiotes Build., 1st Floor, Biotechnology Dept., Physics Lab
DESCRIPTION:  

Euromex Z-range zoom stereo microscope equipped with Cold light source LE.5210 apparatus (Euromex) and digital camera canon EOS3500.


FUNDER:  
 
 
EQUIPMENT UNIT:  
FOC 225i by VELP Scientifica
TYPE:   Refrigerated Incubator
ADDITIONAL INFO:  
 
LOCATION:   75 Iera Odos, Athens 118 55, Greece, Agricultural University of Athens, Hassiotes Build., 1st Floor, Biotechnology Dept., Physics Lab
DESCRIPTION:  
FUNDER:   Physics Lab, AUA
 
 
RELEVANT EXPERTISE:  
UNIVERSITY OF CRETE
LOCATION:   Heraklion, Crete | http://en.uoc.gr/
RELEVANT STAFF:   Kathrin Kefala
SERVICE DETAILS:  

The Douglas OryXNano crystallization robot provides the opportunity of screening a great range of crystallization conditions saving time and sample quantity. Oryx system uses multi-bore dispensing tips (microtips), which have several independent channels to dispense small volumes. At the end of a 3-bore tip there are three holes. Each channel dispenses a different solution. The solutions do not mix in the tip - they mix in the drop after they are dispensed. This means that there is no dead-volume.The microtip always touches the plate when liquids are dispensed.  This gives very reliable dispensing. Stock solutions do not come into contact with the motorized syringes, which are filled with degassed pure water - this avoids the need for flushing out the syringes when the stock solutions are changed.The manual syringes on the front panel are used to refill the motorized syringes, to remove air bubbles, and to load stock solutions for optimization experiments.Screening for sitting drop is carried out using plates that are prefilled with reservoir solutions.  (Reservoirs can be filled manually with a 12-channel pipette.  A very simple user-interface is used to design experiments, as shown below.  The correct plate can be selected from a database of all well-known crystallization plates (if your favorite isn't there, let us know!), the volumes of each ingredient of a drop are entered in a simple form. Individual wells can be selected or skipped by clicking on the plate with a mouse.

RELEVANT EQUIPMENT:  
EQUIPMENT UNIT:  
Robot system crystallization by Douglas Instruments
TYPE:  
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