The baculovirus expression system is commonly used to generate recombinant proteins in insect cells at high production levels. Recombinant baculoviruses can accommodate large segments of foreign DNA. Baculovirus is an established tool for the production of a range of recombinant proteins and multi-protein complexes including virus-like particles
Plasmid design. Bacmid generation. Baculovirus generation and amplification. Small-scale test expression. Medium-scale protein expression and purification.
CO2 incubator
Fast Protein Liquid Chromatography
Large scale production of recombinant proteins expressed by the Baculovirus Expression systems.
For the construction of recombinant baculovirus our service can provide experimental protocols and advices.
BIOBASE Biochemistry incubator, BIOBASE Biological Safety Cabinet Class II A2, Euromex Inverted microscope (OX.2053-PLP, euromex)
CO2 incubator
Fast Protein Liquid Chromatography
Dr. Vassilis Douris is Assistant Professor of Molecular Biology at the University of Ioannina, Department of Biological Applications and Technology. His research team focuses on the analysis of synergistic interactions between molecular mechanisms of insecticide resistance using reverse genetics (CRISPR/Cas9) approaches and functional expression in Drosophila and insect cell-based models, with current emphasis on the potential role of insect chemosensory proteins in insecticide detoxification. His research includes the functional characterization of resistance mutations and detox genes from resistant insects and their employment towards generation of screening platforms for inhibitory compounds to be used as potential synergists (https://www.bri.forth.gr/douris).
Dr. Katerina R. Katsani is Assistant Professor at the Dept of Molecular Biology & Genetics in the Democritus University of Thrace. Dr. Katsani holds a Chemistry degree and a Μaster degree from the Dept of Chemistry of the Aristroteleion University in Thessaloniki. She did her PhD in London (CRUK, former ICRF) and Leiden University Medical Center in the Netherlands from where she obtained her PhD in 2002. During her PhD, she studied chromatin regulators in Drosophila. She then moved to Institut Curie in Paris as a Human Frontiers (HFSPO) post doctoral fellow where she worked on live imaging of nuclear pore complexes. Her main research interests focus on the characterization of the Drosophila nuclear pore complexes, and the investigation of the role of nuclear pore proteins in cell physiology using imaging and proteomic approaches.
BIOBASE Biochemistry incubator, BIOBASE Biological Safety Cabinet Class II A2, Euromex Inverted microscope (OX.2053-PLP, euromex)