Sample preparation  |   Baculovirus expression
 
DESCRIPTION

The baculovirus expression system is commonly used to generate recombinant proteins in insect cells at high production levels. Recombinant baculoviruses can accommodate large segments of foreign DNA. Baculovirus is an established tool for the production of a range of recombinant proteins and multi-protein complexes including virus-like particles

NATIONAL CENTRE FOR SCIENTIFIC RESEARCH "DEMOKRITOS", NCSR-DEMOKRITOS
LOCATION:   Athens | http://www.demokritos.gr/
RELEVANT STAFF:   Georgios Nounesis
SERVICE DETAILS:  

Plasmid design. Bacmid generation. Baculovirus generation and amplification. Small-scale test expression. Medium-scale protein expression and purification.

RELEVANT EQUIPMENT:  
EQUIPMENT UNIT:  
by Heal Force
TYPE:  
ADDITIONAL INFO:  
 
LOCATION:   "Demokritos", Building 9
DESCRIPTION:  

CO2 incubator


FUNDER:   Private operating foundation
 
 
EQUIPMENT UNIT:  
5430R by Eppendorf
TYPE:  
ADDITIONAL INFO:  
 
LOCATION:   "Demokritos", Building 9
DESCRIPTION:  
FUNDER:   ESPA
 
 
EQUIPMENT UNIT:  
Start by AKTA
TYPE:  
ADDITIONAL INFO:  
 
LOCATION:   "Demokritos", Building 9
DESCRIPTION:  

Fast Protein Liquid Chromatography


FUNDER:   Private operating foundation
 
 
RELEVANT EXPERTISE:  
HELLENIC PASTEUR INSTITUTE
LOCATION:   Athens, Greece | https://www.pasteur.gr/en/
RELEVANT STAFF:   Urania Georgopoulou
SERVICE DETAILS:  

Large scale production of recombinant proteins expressed by the Baculovirus Expression systems.

For the construction of recombinant baculovirus our service can provide experimental protocols and advices.

RELEVANT EQUIPMENT:  
EQUIPMENT UNIT:  
Heracell 150 by Thermo Electron
TYPE:   CO2 Incubator
ADDITIONAL INFO:  
 
LOCATION:   Building 8 (1st floor) HPI
DESCRIPTION:  
FUNDER:   HPI
 
 
EQUIPMENT UNIT:  
Optima LE-80K by BECKMAN COULTER
TYPE:   Ultracentrifuge system
ADDITIONAL INFO:  
 
LOCATION:   Building 8 (1st floor) HPI
DESCRIPTION:  
FUNDER:   HPI
 
 
EQUIPMENT UNIT:  
STG-40 by Precision
TYPE:   Baculovirus incubator - 27 C
ADDITIONAL INFO:  
 
LOCATION:   Building 8 (1st floor) HPI
DESCRIPTION:  
FUNDER:   HPI
 
 
EQUIPMENT UNIT:  
Herasafe KS by Thermo Electron
TYPE:   Vertical Laminar Flow Cabinet
ADDITIONAL INFO:  
 
LOCATION:   Building 8 (1st floor) HPI
DESCRIPTION:  
FUNDER:   HPI
 
 
RELEVANT EXPERTISE:  
DEMOCRITUS UNIVERSITY OF THRACE
LOCATION:   Komotini | http://duth.gr/
RELEVANT STAFF:   Katerina Katsani
SERVICE DETAILS:   Not available.
RELEVANT EQUIPMENT:  
EQUIPMENT UNIT:  
incubator BJPX-B250III/ Biological Safety Cabinet BSC-1300IIA2-X/ Inverted microscope OX.2053-PLPH by BIOBASE, EUROMEX
TYPE:   BASIC EQUIPMENT FOR CULTURING INSECT CELLS (baculovirus expression system)
ADDITIONAL INFO:  
 
LOCATION:   DEPARTMENT OF MOLECULAR BIOLOGY AND GENETICS, DEMOCRITUS UNIVERSITY OF THRACE
DESCRIPTION:  

BIOBASE Biochemistry incubator, BIOBASE Biological Safety Cabinet Class II A2,   Euromex  Inverted microscope (OX.2053-PLP, euromex)


FUNDER:   INSPIRED -RIS
 
 
RELEVANT EXPERTISE: