Sample preparation  |   Bacterial expression
 
DESCRIPTION
The description of this service is not available.
NATIONAL HELLENIC RESEARCH FOUNDATION (N.H.R.F.)
LOCATION:   Athens, Greece | http://www.eie.gr/index-en.html
RELEVANT STAFF:   Spyros Zographos , Evangelia Chrysina
SERVICE DETAILS:  

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RELEVANT EQUIPMENT:  
EQUIPMENT UNIT:  
ÄKTApurifier UPC 10 by GE Healthcare
TYPE:   Fast Liquid Protein Chromatography apparatus
ADDITIONAL INFO:  
 
LOCATION:   Institute of Chemical Biology- Laboratory of Structural Biology / Instruct-EL hub
DESCRIPTION:   Fast Liquid Chromatography apparatus for protein purification
FUNDER:   OPERATIONAL PROGRAMME "COMPETITIVENESS", Measure 4.5., Action 4.5.1.: INFRASTRUCTURE TO SUPPORT THE LONG TERM VIABILITY OF RESEARCH ACTIVITIES, TECHNOLOGICAL PROMOTION AND DISSEMINATION. Project title: DESIGN AND SYNTHESIS OF BIOACTIVE COMPOUNDS; FP7-REGPOT-2009-1, No245866, "ARCADE"
 
 
RELEVANT EXPERTISE:  
NATIONAL CENTRE FOR SCIENTIFIC RESEARCH "DEMOKRITOS", NCSR-DEMOKRITOS
LOCATION:   Athens | http://www.demokritos.gr/
RELEVANT STAFF:   Georgios Nounesis , Angeliki Chroni
SERVICE DETAILS:   Not available.
RELEVANT EQUIPMENT:  
EQUIPMENT UNIT:  
Purifier by Acta
TYPE:   FPLC
ADDITIONAL INFO:  
 
LOCATION:   NCSRD, Building 20
DESCRIPTION:  
FUNDER:  
 
 
EQUIPMENT UNIT:  
Eπωαστικός θάλαμος by Labtech
TYPE:  
ADDITIONAL INFO:  
 
LOCATION:   "Demokritos", Building 9 & Building 20
DESCRIPTION:  
FUNDER:   EU
 
 
RELEVANT EXPERTISE:  
HELLENIC PASTEUR INSTITUTE
LOCATION:   Athens, Greece | https://www.pasteur.gr/en/
SERVICE DETAILS:  

Low-cost prokaryotic system for the expression of recombinant intracellular, periplasmic or secreted soluble proteins. The lysis of bacterial cell walls is accomplished either by ultrasound treatment or by the "freeze-thaw" method. The subsequent isolation of the recombinant proteins is achieved through chromatographic analysis.

RELEVANT EQUIPMENT:  
EQUIPMENT UNIT:  
Pellicon 2 by Millipore
TYPE:   Tangential flow filtration cassette
ADDITIONAL INFO:  
 
LOCATION:   Building 6 (groundfloor) HPI
DESCRIPTION:  
FUNDER:  
 
 
EQUIPMENT UNIT:  
Sonicator 3000 by MISONIX
TYPE:   Sonicator
ADDITIONAL INFO:  
 
LOCATION:   Building 6 (groundfloor) HPI
DESCRIPTION:  
FUNDER:  
 
 
EQUIPMENT UNIT:  
Soniprep 150 by MSE
TYPE:   Sonicator
ADDITIONAL INFO:  
 
LOCATION:   Building 8 (3rd floor) HPI
DESCRIPTION:  
FUNDER:  
 
 
EQUIPMENT UNIT:  
Vivaflow 200 by Sartorius
TYPE:   Tangential flow filtration cassette
ADDITIONAL INFO:  
 
LOCATION:   Building 6 (groundfloor) HPI
DESCRIPTION:  
FUNDER:   Inspired
 
 
EQUIPMENT UNIT:  
INR 200 by Gallenkamp
TYPE:   Orbital Shaker
ADDITIONAL INFO:  
 
LOCATION:   Building 8 (3rd floor) HPI
DESCRIPTION:   Bacterial cultures
FUNDER:  
 
 
EQUIPMENT UNIT:  
Sorvall RC-5B by DuPont Instruments
TYPE:   Centrifugal Unit for bacteria
ADDITIONAL INFO:  
 
LOCATION:   Building 8 (3rd floor) HPI
DESCRIPTION:   Bacteria centrifuge
FUNDER:   HPI
 
 
EQUIPMENT UNIT:  
5810R by Eppendorf
TYPE:   Centrifugal Unit for bacteria
ADDITIONAL INFO:  
 
LOCATION:   Building 6 (groundfloor) HPI
DESCRIPTION:   Bacteria centrifuge
FUNDER:  
 
 
EQUIPMENT UNIT:  
Forma Orbital Shaker 420 by Thermo Electron
TYPE:   Refrigerated orbital shaker
ADDITIONAL INFO:  
 
LOCATION:   Building 6 (groundfloor) HPI
DESCRIPTION:  

Bacterial cultures


FUNDER:  
 
 
RELEVANT EXPERTISE:  
AGRICULTURAL UNIVERSITY OF ATHENS
LOCATION:   Athens | https://www2.aua.gr/en
RELEVANT STAFF:   Elias Eliopoulos
SERVICE DETAILS:  

Low-cost prokaryotic system for the expression of recombinant proteins. Small-scale bacterial cultures. 

Visit the provider's website: http://inspired.aua.gr/instruct/

RELEVANT EQUIPMENT:  
RELEVANT EXPERTISE:  
UNIVERSITY OF IOANNINA
LOCATION:   Ioannina, Greece | https://www.uoi.gr/en/
RELEVANT STAFF:   Katerina Soupsana , Anastasia Politou
SERVICE DETAILS:   Not available.
RELEVANT EQUIPMENT:  
EQUIPMENT UNIT:  
Recombinant protein expression in bacterial systems by
TYPE:   Recombinant protein expression in bacterial systems
ADDITIONAL INFO:  
 
LOCATION:   Laboratory of Biological Chemistry, Faculty of Medicine, University of Ioannina, Ioannina, Greece
DESCRIPTION:   The facility offers expertise in recombinant protein expression in E. coli-based bacterial systems. It is equipped with a programmable incubator capable of growth conditions in the temperature range 4-80 degrees, Beckman Avanti centrifuges, a liquid chromatography system (FPLC AKTA purifier, GE Lifesciences), gel electrophoresis and blotting equipment, a sonicator and a French Press for cell disruption, several ultrafiltration systems (Amicon), and a range of columns and resins for protein purification according to size, charge, hydrophobicity, or substrate affinity.
FUNDER:  
 
 
EQUIPMENT UNIT:  
Innova 42R by New Brunswick
TYPE:   Refrigerated orbital shaker
ADDITIONAL INFO:  
 
LOCATION:   Laboratory of Biological Chemistry, Faculty of Medicine, University of Ioannina, Ioannina, Greece
DESCRIPTION:   Temperature range 4-80 degrees with universal platform, 1L and 2L clamps, test-tube rack and sticking tap
FUNDER:   University of Ioannina structural funds
 
 
RELEVANT EXPERTISE: