Biophysical characterization  |   (micro)Calorimetry
 
DESCRIPTION
The description of this service is not available.
NATIONAL CENTRE FOR SCIENTIFIC RESEARCH "DEMOKRITOS", NCSR-DEMOKRITOS
LOCATION:   Athens | http://www.demokritos.gr/
RELEVANT STAFF:   Georgios Nounesis
SERVICE DETAILS:   Not available.
RELEVANT EQUIPMENT:  
EQUIPMENT UNIT:  
VP-DSC by Microcal
TYPE:   Adiabatic DSC
ADDITIONAL INFO:  
 
LOCATION:   Biomolecular Physics Laboratory
DESCRIPTION:  
FUNDER:  
 
 
EQUIPMENT UNIT:  
MCS ITC by Microcal
TYPE:   Isothermal Titration Calorimeter
ADDITIONAL INFO:  
 
LOCATION:   Biomolecular Physics Laboratory
DESCRIPTION:  
FUNDER:  
 
 
RELEVANT EXPERTISE:  
UNIVERSITY OF THESSALY
LOCATION:   Larissa, Karditsa Thessaly, Greece | https://www.uth.gr
RELEVANT STAFF:   George Kontopidis
SERVICE DETAILS:  

Nano Isothermal Titration Calorimeter  from TA instrument (Karditsa)

Isothermal titration calorimetry (ITC) can determine all the thermodynamic parameters (affinity, enthalpy and stoichiometry) of a binding interaction (e.g. A + B ↔AB) in one experiment. 1. ITC works by titrating reactant B into reactant A under isothermal conditions.

User should supply a sample usually purified protein (reactant A) and a ligand (reactant B)

Requirement

Reactant A

Purity > 90%

Concentration: ideally 30 * Kd (e.g. for a Kd around 0.5 μΜ sample concentration should be 15μM around)

Usually working with concentration between 10-30μM

Volume: 1.2ml (ideally for a sample and blank experiments 2.5 ml)

Reactant B

Concentration: 15 to 20 * n * C where n is the stoichiometry of the reaction (A + B ↔AB) where C concentration of reactant A

Volume: 0.1ml (ideally for a sample and blank experiments 0.2 ml)

RELEVANT EQUIPMENT:  
EQUIPMENT UNIT:  
by TA instrument
TYPE:  
ADDITIONAL INFO:  
 
LOCATION:  
DESCRIPTION:  
FUNDER:  
 
 
RELEVANT EXPERTISE: