The 3D structure determination of proteins using X-ray Diffraction is one of the core services of our Infrastructure. Knowledge of the 3D structure of macromolecular targets of pharmaceutical and biotechnological interest and their complexes with small molecules or other proteins may reveal the structure-function relationships that dictate their mechanism of action. Proteins participate in a plethora of metabolic pathways and controlling their interactions with their counterparts or bioactive compounds may allow the development of therapeutic interventions with significant socioeconomic impact.
Potential users: Scientists working in academic/research organizations, health sciences, hospitals, industries (pharmaceutical, food, agricultural, biofuel, paper, chemical) and SMEs.
Instruct-EL hub offers integrated structural biology services using the available infrastructure at NHRF and jointly with partner research infrastructures. It operates In close cooperation with research groups at NHRF specialized in the fields of computational chemistry, organic synthesis, biology, biotechnology and medicine. The quality of the samples prior to crystallization and exposure of the crystals to X-rays may be tested by biophysical methods.
Selected structural analysis services: X-ray diffraction data collection from protein crystals; Preliminary characterization of protein crystals; 3D structure determination of proteins; Structure-based design of potential drugs, therapeutic agents, ligands; Structural analysis from diffraction data collected at large scale facilities.