Dynamic light scattering studies of protein aqueous solutions provide valuable insight regarding protein physicochemical properties such as their molecular size, the existence of different populations (oligomeric protein aggregates) in solution and their size distribution, their hydrodynamic radius and diffusion behaviour. Furthermore, the determination of the molecular weight and radius of gyration, as well as the extraction of information about protein-solvent interactions and molecular shape is also possible by means of multi-angle static light scattering measurements. In addition, light scattering is one of the suitable techniques for studying protein interaction/complexation with other proteins, ligands or biomacromolecules, as well as their aggregation in response to external stimuli like temperature, pH, ionic strength, etc.
