Hellenic Pasteur Institute  |   Mammalian expression
 
DESCRIPTION
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Hellenic Pasteur Institute
LOCATION:   Athens, Greece | https://www.pasteur.gr/en/
SERVICE DETAILS:  

High-yield expression system based on transfected mammalian cells for the production of recombinant proteins with complex post-translational modifications. Apart from the available adherent cells, our Institute has the expertise in using the highly sophisticated system of mammalian Expi293F cells which ensures rapid and high-yield protein production from high density suspension culture. The culture's supernatant is filtrated and then concentrated/dialysed by use of tangential flow filtration systems. The subsequent isolation of the recombinant proteins is achieved through chromatographic analysis.

RELEVANT EQUIPMENT:  
EQUIPMENT UNIT:  
Pellicon 2 by Millipore
TYPE:   Tangential flow filtration cassette
ADDITIONAL INFO:  
 
LOCATION:   Building 6 (groundfloor) HPI
DESCRIPTION:  
FUNDER:  
 
 
 
EQUIPMENT UNIT:  
Vivaflow 200 by Sartorius
TYPE:   Tangential flow filtration cassette
ADDITIONAL INFO:  
 
LOCATION:   Building 6 (groundfloor) HPI
DESCRIPTION:  
FUNDER:   Inspired
 
 
 
EQUIPMENT UNIT:  
Heracell 150 by Thermo Electron
TYPE:   CO2 Incubator
ADDITIONAL INFO:  
 
LOCATION:   Building 6 (groundfloor) HPI
DESCRIPTION:  
FUNDER:  
 
 
 
EQUIPMENT UNIT:  
LVC-5A1 by ESCO
TYPE:   Vertical Laminar Flow Cabinet
ADDITIONAL INFO:  
 
LOCATION:   Building 6 (groundfloor) HPI
DESCRIPTION:  
FUNDER:  
 
 
 
EQUIPMENT UNIT:  
TOS-3530CO2 by MRC
TYPE:   Orbital Shaker
ADDITIONAL INFO:  
 
LOCATION:   Laboratory of Molecular Biology and Immunobiotechnology
DESCRIPTION:  
FUNDER:  
 
 
 
EQUIPMENT UNIT:  
YY3009000 XX6700P10 by Millipore
TYPE:   Pressure filtration system
ADDITIONAL INFO:  
 
LOCATION:   Building 6 (groundfloor) HPI
DESCRIPTION:  
FUNDER:  
 
 
 
RELEVANT EXPERTISE: