STAMATIOS (AKIS) LIOKATIS
Address: Sikelianou 3a, 45221 Ioannina Tel: +30 6976615407
Email: aliokatis@gmail.com
Research positions / experience
Faculty of Medicine, University of Ioannina (Post-Doc - Group leader: Prof. A. Politou) 2023-todate
Leibniz Institute of Molecular Pharmacology (FMP Berlin) Principal Investigator 2014 - 2019
Leibniz Institute of Molecular Pharmacology (FMP Berlin) Post-Doc - In-Cell NMR group (Group leader: Dr. P.Selenko) 2008 – 2013
EMBL-Heidelberg Visiting Ph.D. student – Biomolecular NMR group (Group leader: Prof. M.Sattler) 2005-6-5 months
• Biocenter, University of Basel, Switzerland (Visiting Ph.D. student – M.E. Müller Institute for Struct. Biol. (Group leader: Prof. U.Aebi) 2004
Higher Education
University of Ioannina, Greece (Medical School), Ph.D. in Biochemistry/Structural Biology. Thesis: Structural and functional characterization of the LBR Tudor domain (Supervisor: Anastasia Politou) 2008
University of Ioannina, Greece (Chemistry Department) M.Sc. in Biochemistry Thesis: The impact of intravenous lipid administration in patients with acute lung injury (Supervisor: Marilena Lekka) 2002
University of Ioannina, Greece (Chemistry Department) B.Sc. in Chemistry 1999
Skills - Techniques
• Protein biochemistry/Molecular biology
Bacteria and mammalian cell culture
Cell extracts preparation
Recombinant DNA technology (cloning, PCR, protein overexpression, isotope-labeling)
Protein purification with chromatographic methods (affinity, ion exchange, size exclusion) in batch or automated setup (FPLC/HPLC, AKTA systems)
In vitro reconstitution of nucleosomes
Enzymatic assays (western blotting)
Protein-protein interactions – PPIs (IPs, pull-down assays, analytical gel filtration)
• Protein biophysics/Structural biology
CD spectroscopy (low-resolution structural characterization of proteins)
Electron microscopy – negative staining (Structural characterization of proteins)
ITC (studying PPIs)
Fluorescence spectroscopy (studying PPIs)
NMR spectroscopy (atomic-resolution protein structure, dynamic characterization, enzymatic activities, studying PPIs)
• Software tools
NMR spectroscopy (TopSpin, iNMR, Sparky,CcpNmr)
Molecular graphics (Pymol)
Bionformatic tools (Expasy portal)
Scientific graphing (GraphPad Prism)
Adobe suites (Photoshop, Illustrator)
Office suites (Powerpoint, Word, Excel)
Fellowships / Grants Year
Research grant for a principal investigator from the German Research Foundation (DFG) 2017 Title: Cross-regulation of post-translational protein modifications and effects on the histone H3tail conformations/dynamics: A structural and mechanistic study on whole nucleosomes by NMR spectroscopy – Part Two (240.000 € for 2.5 years).
Research grant for a principal investigator from the German Research Foundation (DFG) 2013 Title: Cross-regulation of post-translational protein modifications and effects on the histone H3tail conformations/dynamics: A structural and mechanistic study on whole nucleosomes by NMR spectroscopy – Part One (295.300 € for 3 years).
FEBS fellowship Jun 2006 Attending the young scientist forum and FEBS congress in Istanbul, Turkey
EMBO short-term Sep-Dec 2004 Training on electron microscopy and analytical ultracentrifugation at the Biocenter of University of Basel, studying the oligomerization state of Lamin B Receptor.
Original publications (full list)
1. Liokatis S. Reconstitution of nucleosomes with differentially isotope-labeled sister histones. J Vis Exp (2017); 121:55349
2. Liokatis S*, Klingberg R, Tan S, Schwarzer D. Differentially Isotope-Labeled Nucleosomes to Study Asymmetric Histone Modification Crosstalk by Time-Resolved NMR Spectroscopy. Angew Chem Int Ed (2016); 55(29):8262-5
3. Stuetzer A*, Liokatis S*, Kiesel A, Schwarzer D, Sprangers R, Soeding J, Selenko P, Fischle W. Modulations of DNA Contacts by Linker Histones and Post-translational Modifications Determine the Mobility and Modifiability of Nucleosomal H3 Tails. Molecular Cell (2016); 61(2):247-59
4. Theillet FX, Rose HM, Liokatis S, Binolfi A, Thongwichian R, Stuiver M, Selenko P. Site-specific mapping and time-resolved NMR monitoring of serine and threonine phosphorylation in reconstituted kinase reaction mixtures or mammalian cell extracts. Nature Protocols (2013);8(7):1416-32
5. Liokatis S, Stuetzer A, Elsaesser SJ, Theillet FX, Klingberg R, van Rossum B, Schwarzer D, Allis CD, Fischle W, Selenko P. Phosphorylation of histone H3 Ser10 establishes a hierarchy for subsequent intramolecular modification events. Nature Struct Mol Biol (2012);19(8):819-23
6. Theillet FX, Liokatis S, Jost JO, Bekei B, Rose HM, Binolfi A, Schwarzer D, Selenko P. Site-specific mapping and time-resolved monitoring of lysine methylation by high-resolution NMR spectroscopy. J Am Chem Soc (2012);134(18):7616-9
7. Theillet FX, Smet-Nocca C, Liokatis S, Thongwichian R, Kosten J, Yoon MK, Kriwacki RW, Landrieu I, Lippens G, Selenko P. Cell signaling, post-translational protein modifications and NMR spectroscopy. J Biomol NMR (2012);54(3):217-36
8. Liokatis S, Edlich C, Soupsana K, Giannios I, Panagiotidou P, Tripsianes K, Sattler M, Georgatos SD, Politou AS. Solution structure and molecular interactions of the Lamin B Receptor Tudor domain. J Biol Chem (2012);287(2):1032-1042
9. Theillet FX, Binolfi A, Liokatis S, Verzini S, Selenko P. Paramagnetic relaxation enhancement to improve sensitivity of fast NMR methods: application to intrinsically disordered proteins. J Biomol NMR (2011);51(4):487-95
10. Dose A*, Liokatis S*, Theillet FX, Schwarzer D, Selenko P. NMR profiling of histone deacetylase and acetyl-transferase activities in real time. ACS Chem Biol (2011);6(5):419
11. Liokatis S, Dose A, Schwarzer D, Selenko P. Simultaneous detection of protein phosphorylation and acetylation by high-resolution NMR spectroscopy. J Am Chem Soc (2010);132(42):14704
12. Karetsou Z, Emmanouilidou A, Sanidas I, Liokatis S, Nikolakaki E, Politou AS, Papamarcaki T. Identification of distinct SET/TAF-Ibeta domains required for core histone binding and quantitative characterisation of the interaction. BMC Biochem (2009):10
13. Lekka ME, Liokatis S, Nathanail C, Galani V, Nakos G. The impact of intravenous fat emulsion administration in acute lung injury. Am J Respir Crit Care Med (2004);169(5):638-44
14. Nakos G, Tsangaris H, Liokatis S, Kitsiouli E, Lekka ME. Ventilator-associated pneumonia and atelectasis: evaluation through bronchoalveolar lavage fluid analysis. Intensive Care Med (2003);29(4):555-63
Conferences (selected)
Liokatis S, Selenko P. Independent observation of sister histones and readout of asymmetric post- translational modifications (PTM) patterns in nucleosomes by high-resolution Nuclear Magnetic Resonance (NMR) spectroscopy (Poster presentation). EMBO Conference: Chromatins and Epigenetics, 2015 – Heidelberg (DE).
Liokatis S, Selenko P. NMR-monitoring of asymmetric post-translational modification patterns in nucleosomes (Oral presentation). 6th Conference of the Hellenic Society of Biochemistry and Molecular Biology: 2015 – Athens (GR).
Liokatis S. Reading asymmetric post-translational modification (PTM) patterns in nucleosomes by high-resolution NMR spectroscopy (Oral presentation). SEE-DRUG Conference- NMR applications in life sciences: 2015 – Patras (GR).
Liokatis S, Stuetzer A, Sprangers R, Schwarzer D, Fischle W, Selenko P. Modulation of transient histone-tail/DNA interactions by post-translational modifications determine the nucleosomal histone H3 code (Poster presentation). EMBO Workshop on Magnetic resonance for cellular structural biology: 2014 – Grosseto (IT).
Liokatis S, Stuetzer A, Elsaesser SJ, Allis CD, Sprangers R, Fischle W, Selenko P. A ta(i)le of post- translational modifications and dynamics (Oral presentation). Chromatin and Systems Biology, Summer School: 2011 – Spetses (GR).
Liokatis S, Edlich C, Sattler M, Georgatos SD, Politou AS. Structural and functional characterization of Lamin B Receptor (LBR) Tudor domain (Poster presentation). FEBS Biochemistry Congress: 2006 – Istanbul (TR).
Liokatis S, Makatsori D, Sattler M, Georgatos SD, Politou AS. Structural-functional studies of the Lamin B Receptor (LBR) (Poster presentation). EMBL Conference- Structures in Biology: 2004 – Istanbul (TR).
Languages
Greek (Mother tongue)
English (Fluent writing and spoken) – Proficiency from University of Michigan
German (B2 level communication skills)
