Sample preparation  |   Yeast expression
 
DESCRIPTION
The description of this service is not available.
HELLENIC PASTEUR INSTITUTE
LOCATION:   Athens, Greece | https://www.pasteur.gr/en/
SERVICE DETAILS:  

Low-cost eukaryotic system (methylotrophic yeast Pichia pastoris) for the large-scale expression of recombinant proteins. The lysis of yeast cells is accomplished either by ultrasound treatment or mechanical methods. In case of soluble recombinant protein expression, the culture's supernatant is concentrated/dialysed by use of tangential flow filtration systems. The subsequent isolation of the recombinant proteins is achieved through chromatographic analysis.

RELEVANT EQUIPMENT:  
EQUIPMENT UNIT:  
Pellicon 2 by Millipore
TYPE:   Tangential flow filtration cassette
ADDITIONAL INFO:  
 
LOCATION:   Building 6 (groundfloor) HPI
DESCRIPTION:  
FUNDER:  
 
 
EQUIPMENT UNIT:  
Vivaflow 200 by Sartorius
TYPE:   Tangential flow filtration cassette
ADDITIONAL INFO:  
 
LOCATION:   Building 6 (groundfloor) HPI
DESCRIPTION:  
FUNDER:   Inspired
 
 
EQUIPMENT UNIT:  
SH-20C by Χρυσάγης
TYPE:   P. pastoris incubator
ADDITIONAL INFO:  
 
LOCATION:   Building 6 (groundfloor) HPI
DESCRIPTION:   P. pastoris cultures
FUNDER:   HPI
 
 
EQUIPMENT UNIT:  
LM-530-2 by Mrc
TYPE:   P. pastoris incubator
ADDITIONAL INFO:  
 
LOCATION:   Building 6 (groundfloor) HPI
DESCRIPTION:  
FUNDER:  
 
 
EQUIPMENT UNIT:  
Rotina 420R by Hettich
TYPE:   Centrifugal Unit for Pichia pastoris
ADDITIONAL INFO:  
 
LOCATION:   Building 6 (2nd floor) HPI
DESCRIPTION:  
FUNDER:  
 
 
EQUIPMENT UNIT:  
YY3009000 XX6700P10 by Millipore
TYPE:   Pressure filtration system
ADDITIONAL INFO:  
 
LOCATION:   Building 6 (groundfloor) HPI
DESCRIPTION:  
FUNDER:  
 
 
EQUIPMENT UNIT:  
7780 by KUBOTA
TYPE:   Centrifugal Unit for P.pastoris
ADDITIONAL INFO:  
 
LOCATION:   Building 6 (groundfloor) HPI
DESCRIPTION:   P. pastoris centrifugal unit
FUNDER:  
 
 
RELEVANT EXPERTISE:  

The only available yeast for the expression of recombinant proteins is the methylotrophic Pichia pastoris.