No CV provided

 

STAMATIOS (AKIS) LIOKATIS

Address: Sikelianou 3a, 45221 Ioannina Tel: +30 6976615407
Email: aliokatis@gmail.com

Research positions / experience

Faculty of Medicine, University of Ioannina (Post-Doc - Group leader: Prof. A. Politou)           2023-todate

Leibniz Institute of Molecular Pharmacology (FMP Berlin) Principal Investigator                     2014 - 2019

Leibniz Institute of Molecular Pharmacology (FMP Berlin) Post-Doc - In-Cell NMR group (Group leader: Dr. P.Selenko) 2008 – 2013

EMBL-Heidelberg Visiting Ph.D. student – Biomolecular NMR group (Group leader: Prof. M.Sattler) 2005-6-5 months

• Biocenter, University of Basel, Switzerland (Visiting Ph.D. student – M.E. Müller Institute for Struct. Biol. (Group leader: Prof. U.Aebi)  2004

Higher Education

University of Ioannina, Greece (Medical School), Ph.D. in Biochemistry/Structural Biology. Thesis: Structural and functional characterization of the LBR Tudor domain (Supervisor: Anastasia Politou) 2008

University of Ioannina, Greece (Chemistry Department) M.Sc. in Biochemistry Thesis: The impact of intravenous lipid administration in patients with acute lung injury (Supervisor: Marilena Lekka) 2002

University of Ioannina, Greece (Chemistry Department) B.Sc. in Chemistry 1999

Skills - Techniques

 

• Protein biochemistry/Molecular biology
Bacteria and mammalian cell culture
Cell extracts preparation
Recombinant DNA technology (cloning, PCR, protein overexpression, isotope-labeling)
Protein purification with chromatographic methods (affinity, ion exchange, size exclusion) in batch or automated setup (FPLC/HPLC, AKTA systems)

In vitro reconstitution of nucleosomes
Enzymatic assays (western blotting)
Protein-protein interactions – PPIs (IPs, pull-down assays, analytical gel filtration)
• Protein biophysics/Structural biology
CD spectroscopy (low-resolution structural characterization of proteins)
Electron microscopy – negative staining (Structural characterization of proteins)
ITC (studying PPIs)
Fluorescence spectroscopy (studying PPIs)
NMR spectroscopy (atomic-resolution protein structure, dynamic characterization, enzymatic activities, studying PPIs)
• Software tools
NMR spectroscopy (TopSpin, iNMR, Sparky,CcpNmr)
Molecular graphics (Pymol)
Bionformatic tools (Expasy portal)
Scientific graphing (GraphPad Prism)
Adobe suites (Photoshop, Illustrator)
Office suites (Powerpoint, Word, Excel)

Fellowships / Grants Year

Research grant for a principal investigator from the German Research Foundation (DFG) 2017 Title: Cross-regulation of post-translational protein modifications and effects on the histone H3tail conformations/dynamics: A structural and mechanistic study on whole nucleosomes by NMR spectroscopy – Part Two (240.000 € for 2.5 years).

Research grant for a principal investigator from the German Research Foundation (DFG) 2013 Title: Cross-regulation of post-translational protein modifications and effects on the histone H3tail conformations/dynamics: A structural and mechanistic study on whole nucleosomes by NMR spectroscopy – Part One (295.300 € for 3 years).

FEBS fellowship Jun 2006 Attending the young scientist forum and FEBS congress in Istanbul, Turkey

EMBO short-term Sep-Dec 2004 Training on electron microscopy and analytical ultracentrifugation at the Biocenter of University of Basel, studying the oligomerization state of Lamin B Receptor.

Original publications (full list)

1. Liokatis S. Reconstitution of nucleosomes with differentially isotope-labeled sister histones. J Vis Exp (2017); 121:55349

2. Liokatis S*, Klingberg R, Tan S, Schwarzer D. Differentially Isotope-Labeled Nucleosomes to Study Asymmetric Histone Modification Crosstalk by Time-Resolved NMR Spectroscopy. Angew Chem Int Ed (2016); 55(29):8262-5
3. Stuetzer A*, Liokatis S*, Kiesel A, Schwarzer D, Sprangers R, Soeding J, Selenko P, Fischle W.  Modulations of DNA Contacts by Linker Histones and Post-translational Modifications Determine the Mobility and Modifiability of Nucleosomal H3 Tails. Molecular Cell (2016); 61(2):247-59

4. Theillet FX, Rose HM, Liokatis S, Binolfi A, Thongwichian R, Stuiver M, Selenko P. Site-specific mapping and time-resolved NMR monitoring of serine and threonine phosphorylation in reconstituted kinase reaction mixtures or mammalian cell extracts. Nature Protocols (2013);8(7):1416-32

5. Liokatis S, Stuetzer A, Elsaesser SJ, Theillet FX, Klingberg R, van Rossum B, Schwarzer D, Allis CD, Fischle W, Selenko P. Phosphorylation of histone H3 Ser10 establishes a hierarchy for subsequent intramolecular modification events. Nature Struct Mol Biol (2012);19(8):819-23

6. Theillet FX, Liokatis S, Jost JO, Bekei B, Rose HM, Binolfi A, Schwarzer D, Selenko P. Site-specific mapping and time-resolved monitoring of lysine methylation by high-resolution NMR spectroscopy. J Am Chem Soc (2012);134(18):7616-9
7. Theillet FX, Smet-Nocca C, Liokatis S, Thongwichian R, Kosten J, Yoon MK, Kriwacki RW,  Landrieu I, Lippens G, Selenko P. Cell signaling, post-translational protein modifications and NMR  spectroscopy. J Biomol NMR (2012);54(3):217-36
8. Liokatis S, Edlich C, Soupsana K, Giannios I, Panagiotidou P, Tripsianes K, Sattler M, Georgatos SD,  Politou AS. Solution structure and molecular interactions of the Lamin B Receptor Tudor domain. J Biol Chem (2012);287(2):1032-1042 

9. Theillet FX, Binolfi A, Liokatis S, Verzini S, Selenko P. Paramagnetic relaxation enhancement to improve sensitivity of fast NMR methods: application to intrinsically disordered proteins. J Biomol NMR (2011);51(4):487-95

10. Dose A*, Liokatis S*, Theillet FX, Schwarzer D, Selenko P. NMR profiling of histone deacetylase and acetyl-transferase activities in real time. ACS Chem Biol (2011);6(5):419

11. Liokatis S, Dose A, Schwarzer D, Selenko P. Simultaneous detection of protein phosphorylation and  acetylation by high-resolution NMR spectroscopy. J Am Chem Soc (2010);132(42):14704
12. Karetsou Z, Emmanouilidou A, Sanidas I, Liokatis S, Nikolakaki E, Politou AS, Papamarcaki T.  Identification of distinct SET/TAF-Ibeta domains required for core histone binding and quantitative  characterisation of the interaction. BMC Biochem (2009):10
13. Lekka ME, Liokatis S, Nathanail C, Galani V, Nakos G. The impact of intravenous fat emulsion  administration in acute lung injury. Am J Respir Crit Care Med (2004);169(5):638-44
14. Nakos G, Tsangaris H, Liokatis S, Kitsiouli E, Lekka ME. Ventilator-associated pneumonia and  atelectasis: evaluation through bronchoalveolar lavage fluid analysis. Intensive Care Med (2003);29(4):555-63

Conferences (selected)

 

Liokatis S, Selenko P. Independent observation of sister histones and readout of asymmetric post- translational modifications (PTM) patterns in nucleosomes by high-resolution Nuclear Magnetic Resonance (NMR) spectroscopy (Poster presentation). EMBO Conference: Chromatins and Epigenetics, 2015 – Heidelberg (DE).

Liokatis S, Selenko P. NMR-monitoring of asymmetric post-translational modification patterns in nucleosomes (Oral presentation). 6th Conference of the Hellenic Society of Biochemistry and Molecular Biology: 2015 – Athens (GR).

Liokatis S. Reading asymmetric post-translational modification (PTM) patterns in nucleosomes by high-resolution NMR spectroscopy (Oral presentation). SEE-DRUG Conference- NMR applications in life sciences: 2015 – Patras (GR).

Liokatis S, Stuetzer A, Sprangers R, Schwarzer D, Fischle W, Selenko P. Modulation of transient histone-tail/DNA interactions by post-translational modifications determine the nucleosomal histone H3 code (Poster presentation). EMBO Workshop on Magnetic resonance for cellular structural biology: 2014 – Grosseto (IT).

Liokatis S, Stuetzer A, Elsaesser SJ, Allis CD, Sprangers R, Fischle W, Selenko P. A ta(i)le of post- translational modifications and dynamics (Oral presentation). Chromatin and Systems Biology, Summer School: 2011 – Spetses (GR).

Liokatis S, Edlich C, Sattler M, Georgatos SD, Politou AS. Structural and functional characterization of Lamin B Receptor (LBR) Tudor domain (Poster presentation). FEBS Biochemistry Congress: 2006 – Istanbul (TR).

Liokatis S, Makatsori D, Sattler M, Georgatos SD, Politou AS. Structural-functional studies of the Lamin B Receptor (LBR) (Poster presentation). EMBL Conference- Structures in Biology: 2004 – Istanbul (TR).

Languages

Greek (Mother tongue)

English (Fluent writing and spoken) – Proficiency from University of Michigan

German (B2 level communication skills)

 

No CV provided

Anastasia S. Politou is an Associate Professor of Biological Chemistry at the Ioannina University, Faculty of Medicine and an affiliated faculty member of FORTH-IMBB-BR. She holds a B.Sc. in Chemistry from the University of Athens, Greece and M.Sc. and Ph.D. degrees in Chemistry from New York University, USA. She worked as a post-doctoral fellow in the Structural Biology and Biocomputing Program of the European Molecular Biology Laboratory (EMBL) from 1991 to 1996. From 1996 to 1999 she was a Visiting Associate Professor in the Chemistry Dept., University of Crete and in 2000 a Senior Research Fellow, Medical Research Council, National Institute for Medical Research, Mill Hill, London, UK. Since 2001 she has been a faculty member of the University of Ioannina. A.S. Politou has published 44 research articles (over 2500 citations, h-index:28). Her group is active in the areas of Structural Biology and Biophysics, Computational/Structural Epigenetics and Transcriptomics. The group has been extensively involved in modeling and structural analysis of protein complexes, in bioinformatics analysis of DNA and protein sequences and in the analysis and evaluation of high-throughput gene expression data. Ongoing research focuses on different aspects of epigenetic regulation and aims at characterizing the structural determinants and dynamics of chromatin-related proteins and protein complexes. Over the years, Anastasia Politou has established and managed several tools and facilities that have been widely used by several teams in Greece and abroad. These include the establishment and management of the first High Resolution NMR facility in Greece (University of Crete) and of the Circular Dichroism and Fluorescence Spectroscopy facility at the University of Ioannina and IMBB-BR, which are included in the National Research Infrastructure Program INSPIRED. She is also the coordinator of the Ioannina team in the national (ELIXIR-GR) and the European consortium (ELIXIR-EU) that supports the biological data and management infrastructure ELIXIR.