Production of recombinant proteins in bacterial, yeast or Leishmania expression systems, at large scale in a bioreactor, with the potential for multiple parameter controls.
The available unit (BioFlo 415) is a versatile sterilizable-in-place (SIP) fermentor for total culture volume of 20L. It can be employed for batch or continuous culture with process control of pH, dissolved oxygen (DO), agitation, temperature, pump feed, antifoam, level and addition analog/digital inputs and outputs (up to 32 parameters). Only the heterologous expression systems available at the Hellenic Pasteur Institute (bacteria, Pichia pastoris and Leishmania tarentolae) can be used in the fermentor.